Optogenetics allows scientists to control the activity of specific neurons and study their downstream influence on a variety of biological processes, including behavior. In rodent studies this technique requires optical fiber implantation, but as they are transparent, fiber optics are not needed in studies with zebrafish larvae. Light simply needs to shine in the right direction, making it easy to study the role of specific neurons in behavior.
In zebrafish studies, the light-sensitive receptor protein halorhodopsin (NpHR) has successfully been used to inhibit swimming behavior in zebrafish larvae (Arrenberg, et al., 2009). Other research showed that channelrhodopsin-2 (ChR2) activation induced backward swimming in a sparse transgene expression line (Zhu et al., 2009).
Application of optogenetic stimulation in zebrafish larvae does not require fiber optics, but it does require the correct wavelength (color) of light. As the application of optogenetics in the field of zebrafish neurobiology increases, so does the need for efficient protocols. When using the DanioVision Observation Chamber, the Optogenetics Add-on provides a way to accurately control and precisely time the application of optogenetic stimulation to up to 98 individuals simultaneously (working with 98 well plates).
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